• Product name
  • Description
    Rabbit polyclonal to Rad21
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 6-399 of Human Rad21 (UniProt ID: O60216).

  • Positive control
    • K562, THP1, HL60, NCI-H929, NIH3T3, JC and BCL-1 whole cell lysate; HeLa cells; Human HBL435 and Mouse C2C12 xenograft tissues



Our Abpromise guarantee covers the use of ab154769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.


  • Function
    Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
  • Sequence similarities
    Belongs to the rad21 family.
  • Domain
    The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.
  • Post-translational
    Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
    Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDLS4 antibody
    • Double-strand-break repair protein rad21 homolog antibody
    • hHR21 antibody
    • HR21 antibody
    • HRAD21 antibody
    • KIAA0078 antibody
    • MCD1 antibody
    • Nuclear matrix protein 1 antibody
    • NXP-1 antibody
    • NXP1 antibody
    • Protein involved in DNA double-strand break repair antibody
    • RAD21 antibody
    • RAD21 homolog (S. pombe) antibody
    • RAD21 homolog antibody
    • RAD21_HUMAN antibody
    • Scc1 antibody
    • SCC1 homolog antibody
    see all


  • All lanes : Anti-Rad21 antibody (ab154769) at 1/1000 dilution

    Lane 1 : K562 whole cell lysate
    Lane 2 : THP1 whole cell lysate
    Lane 3 : HL60 whole cell lysate
    Lane 4 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa

    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.
  • Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.
  • All lanes : Anti-Rad21 antibody (ab154769) at 1/3000 dilution

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa

    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.


This product has been referenced in:
  • Biswas U  et al. SMC1a Substitutes for Many Meiotic Functions of SMC1ß but Cannot Protect Telomeres from Damage. Curr Biol 28:249-261.e4 (2018). Read more (PubMed: 29337080) »
  • Srinivasan M  et al. The Cohesin Ring Uses Its Hinge to Organize DNA Using Non-topological as well as Topological Mechanisms. Cell 173:1508-1519.e18 (2018). Read more (PubMed: 29754816) »

See all 9 Publications for this product

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