Product nameAnti-Rad21 antibody
See all Rad21 primary antibodies
DescriptionRabbit polyclonal to Rad21
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Recombinant full length protein (His-tag) corresponding to Schizosaccharomyces pombe Rad21 aa 1-628.
MFYSEAILSK KGPLAKVWLA AHWEKKLSKV QTLHTSIEQS VHAIVTEETA PMALRLSGQL MLGVVRIYSR KARYLLEDCT EALMRLKMSF QPGQVDMIEP ATALQSLKGK DAVTQSANLT LPETITEFDL LVPDSTFDFQ WSQLLRTPSR SSNTLELHSL PISSSPSFPS SQLSIEAGRN AQVESGFSLG ESFAHVGNDM QFHLPISNSG AATPRSVHSD NQSQISIEVG RDAPAAAATD LSGIIGPQMT KSPASSVTHF STPSMLPIGG TSLDDELLAP VDDLNLDLGL DDLLGDEQGA NAPAIEADEQ AETSSIHLPS DIMEDDSSRP AAAGVEEGQV VESATAPQQE KINPQKTVRR QRAIIDPVTE LSSKQMKKQL ADTSSITSPL CLNTSSIVFN ATVNFTRNGK FNTSIFSSNL NPKVNELLQA DFKQAILRKR KNESPEEVEP AKHQRTDTST ENQETAEVLD PEEIAAAELA NITEAAIATL PQETVVQPEG EAPELGSPMG FPVTALESAD DSLFDAPPVM LDEADLLGSE RLDSSVSEAL PSSQTAKDSL RNKWDPYTEG EKVSFQTLSA GCNREEAVQL FFDVLVLATK DVISVKQDVA IQNEITLTAK RGMLLSSL
Database link: P30776
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab171462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/4000. Detects a band of approximately 110 kDa (predicted molecular weight: 68 kDa).|
FunctionCleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Sequence similaritiesBelongs to the rad21 family.
DomainThe C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.
modificationsCleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
- Information by UniProt
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
Lanes 1-2 : Anti-Rad21 antibody (ab171462) at 1/1000 dilution
Lanes 3-4 : Anti-Rad21 antibody (ab171462) at 1/2000 dilution
Lanes 5-6 : Anti-Rad21 antibody (ab171462) at 1/4000 dilution
Lanes 1 & 3 & 5 : WT S. pombe cells
Lanes 2 & 4 : rad21-9xPK S. pombe cells
Lane 6 : rad21-9xPK S. pombe cells
Lane 1 : c at 1/10000 dilution
Lanes 2-6 : anti-rabbit HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
This product has been referenced in:
- Adachi Y et al. Cut1/separase-dependent roles of multiple phosphorylation of fission yeast cohesion subunit Rad21 in post-replicative damage repair and mitosis. Cell Cycle 7:765-76 (2008). Read more (PubMed: 18239448) »
- Nagao K et al. Separase-mediated cleavage of cohesin at interphase is required for DNA repair. Nature 430:1044-8 (2004). Read more (PubMed: 15329725) »