Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: IHC-P, ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Rad21 antibody
See all Rad21 primary antibodies
DescriptionRabbit polyclonal to Rad21
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
- WB: HeLa cell lysate, 293T, NIH3T3. IHC-P: Human breast fibroadenoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.
Used at a dilution of 1/200 for 30 min incubation (see Abreview for further information).
|WB||1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Band observed at ~130 kDa.
|IP||Use a concentration of 1 - 4 µg/ml.|
FunctionCleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Sequence similaritiesBelongs to the rad21 family.
DomainThe C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.
modificationsCleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
- Information by UniProt
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
Anti-Rad21 antibody (ab992) at 1/1000 dilution + HeLa whole cell lysate
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa (possible cross reactivity)
IHC image of Rad21 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab992, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Rad21 antibody (ab992) at 1/2000 dilution + HeLa whole cell extract (extraction was achieved using RIPA buffer and 1% SDS) at 25 µg/ml
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1500 dilution
Predicted band size: 72 kDa
A rad21 knock down negative control was employed.
This image is courtesy of an Abreview submitted on 9 September 2005. We do not have any further information relating to this image.
All lanes : Anti-Rad21 antibody (ab992) at 2 µg/ml
Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Lane 4 : 293T at 50 µg
Lane 5 : NIH3T3 at 50 µg
Predicted band size: 72 kDa
Exposure time: 30 seconds
ab992 has been referenced in 124 publications.
- Kentepozidou E et al. Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains. Genome Biol 21:5 (2020). PubMed: 31910870
- Khoury A et al. Constitutively bound CTCF sites maintain 3D chromatin architecture and long-range epigenetically regulated domains. Nat Commun 11:54 (2020). PubMed: 31911579
- Jia Z et al. Tandem CTCF sites function as insulators to balance spatial chromatin contacts and topological enhancer-promoter selection. Genome Biol 21:75 (2020). PubMed: 32293525
- Lhoumaud P et al. NSD2 overexpression drives clustered chromatin and transcriptional changes in a subset of insulated domains. Nat Commun 10:4843 (2019). PubMed: 31649247
- Yamada T et al. Sensory experience remodels genome architecture in neural circuit to drive motor learning. Nature 569:708-713 (2019). PubMed: 31068695