Recombinant Anti-Rad21 antibody [BLR052F] (ab243905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR052F] to Rad21
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Rad21 antibody [BLR052F]
See all Rad21 primary antibodies -
Description
Rabbit monoclonal [BLR052F] to Rad21 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Rad21 aa 581-631 (C terminal). The exact sequence is proprietary. NP_006256.1 and Gene ID 5885.
Database link: O60216 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR052F -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
Notes |
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WB
1/1000. |
Target
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Function
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
Sequence similarities
Belongs to the rad21 family. -
Domain
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
Post-translational
modificationsCleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Omim: 606462 Human
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
- Unigene: 81848 Human
- Unigene: 182628 Mouse
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Alternative names
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
Images
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Western blot analysis using ab243905 at 1/1000 dilution.
Lane 1: NIH/3T3 whole cell lysate (50 µg).
Lane 2: TCMK-1 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
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Western blot analysis using ab243905 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: Jurkat whole cell lysate (50 µg).
Lane 3: HEK-293T whole cell lysate (50 µg).
Lane 4: RKO whole cell lysate (50 µg).
Lane 5: GaMG whole cell lysate (50 µg).
Lane 6: MCF7 whole cell lysate (50 µg).
Lane 7: A549 whole cell lysate (50 µg).
Lane 8: U-2 OS whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243905 has not yet been referenced specifically in any publications.