Recombinant
RabMAb

Recombinant Anti-Rad21 antibody [EPR12647] - BSA and Azide free (ab241395)

Overview

  • Product name

    Anti-Rad21 antibody [EPR12647] - BSA and Azide free
    See all Rad21 primary antibodies
  • Description

    Rabbit monoclonal [EPR12647] to Rad21 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Rad21 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60216

  • Positive control

    • Flow cyt: K562 cells.
  • General notes

    Ab241395 is the carrier-free version of ab175215. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab241395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab241395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
    • Sequence similarities

      Belongs to the rad21 family.
    • Domain

      The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.
    • Post-translational
      modifications

      Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
      Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • CDLS4 antibody
      • Double-strand-break repair protein rad21 homolog antibody
      • hHR21 antibody
      • HR21 antibody
      • HRAD21 antibody
      • KIAA0078 antibody
      • MCD1 antibody
      • Nuclear matrix protein 1 antibody
      • NXP-1 antibody
      • NXP1 antibody
      • Protein involved in DNA double-strand break repair antibody
      • RAD21 antibody
      • RAD21 homolog (S. pombe) antibody
      • RAD21 homolog antibody
      • RAD21_HUMAN antibody
      • Scc1 antibody
      • SCC1 homolog antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized K562 cells labeling Rad21 with ab175215 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175215).

    References

    ab241395 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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