Overview

  • Product name
    Anti-Rad22 antibody
  • Description
    Rabbit polyclonal to Rad22
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Purified recombinant Rad22 protein (Schizosaccharomyces pombe)

  • Positive control
    • Schizosaccharomyces pombe whole cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Whole serum
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • DNA repair and recombination protein rad22 antibody
      • DNA repair protein Rad22 antibody
      • Rad 22 antibody

    Images

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract

      Predicted band size: 52 kDa

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain

      Predicted band size: 52 kDa
      Observed band size: 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.



      ab63800 was used unpurified.

    References

    ab63800 has not yet been referenced specifically in any publications.

    Publishing research using ab63800? Please let us know so that we can cite the reference in this datasheet.

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