Anti-Rad22 antibody (ab63800)
Key features and details
- Rabbit polyclonal to Rad22
- Suitable for: ELISA, WB, IP, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Overview
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Product name
Anti-Rad22 antibody -
Description
Rabbit polyclonal to Rad22 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Purified recombinant Rad22 protein (Schizosaccharomyces pombe)
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Positive control
- Schizosaccharomyces pombe whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2543086 Schizosaccharomyces pombe
- SwissProt: P36592 Schizosaccharomyces pombe
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Alternative names
- DNA repair and recombination protein rad22 antibody
- DNA repair protein Rad22 antibody
- Rad 22 antibody
Images
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Western blot - Anti-Rad22 antibody (ab63800)Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract
Predicted band size: 52 kDa -
Western blot - Anti-Rad22 antibody (ab63800)All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)
Lane 1 : Molecular weight markers
Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.ab63800 was used unpurified.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab63800 has been referenced in 2 publications.
- Hardy J et al. Histone deposition promotes recombination-dependent replication at arrested forks. PLoS Genet 15:e1008441 (2019). PubMed: 31584934
- Gómez-Escoda B & Wu PJ The organization of genome duplication is a critical determinant of the landscape of genome maintenance. Genome Res 28:1179-1192 (2018). PubMed: 29934426
