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    rad22-antibody-ab63800.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-Rad22 antibody (ab63800)

  • Datasheet
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Western blot - Anti-Rad22 antibody (ab63800)
  • Western blot - Anti-Rad22 antibody (ab63800)

Key features and details

  • Rabbit polyclonal to Rad22
  • Suitable for: ELISA, WB, IP, ICC/IF
  • Reacts with: Schizosaccharomyces pombe
  • Isotype: IgG

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Overview

  • Product name

    Anti-Rad22 antibody
  • Description

    Rabbit polyclonal to Rad22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Purified recombinant Rad22 protein (Schizosaccharomyces pombe)

  • Positive control

    • Schizosaccharomyces pombe whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab63800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 2543086 Schizosaccharomyces pombe
    • SwissProt: P36592 Schizosaccharomyces pombe
    • Alternative names

      • DNA repair and recombination protein rad22 antibody
      • DNA repair protein Rad22 antibody
      • Rad 22 antibody

    Images

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract

      Predicted band size: 52 kDa

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain

      Predicted band size: 52 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.



      ab63800 was used unpurified.

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab63800? Please let us know so that we can cite the reference in this datasheet.

    ab63800 has been referenced in 2 publications.

    • Hardy J  et al. Histone deposition promotes recombination-dependent replication at arrested forks. PLoS Genet 15:e1008441 (2019). PubMed: 31584934
    • Gómez-Escoda B & Wu PJ The organization of genome duplication is a critical determinant of the landscape of genome maintenance. Genome Res 28:1179-1192 (2018). PubMed: 29934426

    Customer reviews and Q&As

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