Anti-Rad22 antibody (ab63800)

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Overview

  • Product name

    Anti-Rad22 antibody

  • Description

    Rabbit polyclonal to Rad22

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Schizosaccharomyces pombe

  • Immunogen

    Purified recombinant Rad22 protein (Schizosaccharomyces pombe)

  • Positive control

    • Schizosaccharomyces pombe whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab63800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • DNA repair and recombination protein rad22 antibody
      • DNA repair protein Rad22 antibody
      • Rad 22 antibody

    Images

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract

      Predicted band size: 52 kDa

    • Western blot - Anti-Rad22 antibody (ab63800)
      Western blot - Anti-Rad22 antibody (ab63800)
      All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain

      Predicted band size: 52 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.



      ab63800 was used unpurified.

    References

    This product has been referenced in:

    • Gómez-Escoda B & Wu PJ The organization of genome duplication is a critical determinant of the landscape of genome maintenance. Genome Res 28:1179-1192 (2018). Read more (PubMed: 29934426) »
    See 1 Publication for this product

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