Anti-Rad50 antibody [13B3/2C6] (ab89)
![Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)](https://www.abcam.com/ps/products/0/ab89/Images/ab89-3371-anti-rad50-antibody-13b3-2c6-western-blot.jpg "Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)")
Key features and details
- Mouse monoclonal [13B3/2C6] to Rad50
- Suitable for: Flow Cyt, IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Rad50 antibody [13B3/2C6]
See all Rad50 primary antibodies -
Description
Mouse monoclonal [13B3/2C6] to Rad50 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Rad50 aa 1-450. Expressed in E.coli.
Database link: Q92878 -
Positive control
- WB: HEK293 total cell lysate. ICC: HeLa cells. IHC-P: Human placenta tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 01/02/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Clone 2C6 recognizes Rad50, a 153kDa protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity purified by Protein G. -
Clonality
Monoclonal -
Clone number
13B3/2C6 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/100 - 1/1000.
|
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| ICC |
1/200.
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| WB | (5) |
1/500 - 1/3000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
|
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/100 - 1/1000. |
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ICC
1/200. |
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WB
1/500 - 1/3000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa). |
Target
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Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
Tissue specificity
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. -
Involvement in disease
Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent. -
Sequence similarities
Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain. -
Domain
The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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Database links
- Entrez Gene: 10111 Human
- Omim: 604040 Human
- SwissProt: Q92878 Human
- Unigene: 633509 Human
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Alternative names
- DNA repair protein RAD50 antibody
- hRAD50 antibody
- hsRAD50 antibody
see all
Images
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Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)
This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.
The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).
This image was generated using the ascites version of the product.
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![Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)](https://www.abcam.com/ps/products/0/ab89/Images/ab89-3370-anti-rad50-antibody-13b3-2c6-western-blot.jpg)
Western blot - Anti-Rad50 antibody [13B3/2C6] (ab89)Anti-Rad50 antibody [13B3/2C6] (ab89) at 1/1000 dilution + 50 ug Human HEK293 total cell lysate
Secondary
HRP conjugated Donkey Anti-Mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 146 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa (possible non-specific binding)
Exposure time: 30 secondsThis image is courtesy of an Abreview submitted by Philippe Szankasi on 24 February 2006.
This image was generated using the ascites version of the product.
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![Immunocytochemistry - Anti-Rad50 antibody [13B3/2C6] (ab89)](https://www.abcam.com/ps/products/0/ab89/Images/ab89-465377-anti-rad50-antibody-13b3-2c6-immunocytochemistry-hela-cells.jpg)
Immunocytochemistry - Anti-Rad50 antibody [13B3/2C6] (ab89)Immunocytochemical analysis of 4% paraformaldehyde-fixed, HeLa cells labelling Rad50 with ab89 at 1/200 dilution (green). Phalloidin at a 1/200 dilution was used to counterstain the cytoskeleton (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [13B3/2C6] (ab89)](https://www.abcam.com/ps/products/0/ab89/Images/ab89-3368-anti-rad50-antibody-13b3-2c6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [13B3/2C6] (ab89)ab89 (1µg/ml) staining RAD50 in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Rad50 antibody [13B3/2C6] (ab89)](https://www.abcam.com/ps/products/0/ab89/Images/ab89-3367-anti-rad50-antibody-13b3-2c6-flow-cytometry.jpg)
Flow Cytometry - Anti-Rad50 antibody [13B3/2C6] (ab89)Overlay histogram showing HeLa cells stained with ab89 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab89 has been referenced in 49 publications.
- Dubich T et al. 3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells. J Mol Med (Berl) 99:425-438 (2021). PubMed: 33484281
- Xu WH et al. Prognostic implication and functional annotations of Rad50 expression in patients with prostate cancer. J Cell Biochem 121:3124-3134 (2020). PubMed: 31886567
- Elkafas H et al. Vitamin D3 Ameliorates DNA Damage Caused by Developmental Exposure to Endocrine Disruptors in the Uterine Myometrial Stem Cells of Eker Rats. Cells 9:N/A (2020). PubMed: 32545544
- Dunlop CR et al. Complete loss of ATM function augments replication catastrophe induced by ATR inhibition and gemcitabine in pancreatic cancer models. Br J Cancer 123:1424-1436 (2020). PubMed: 32741974
- Park H et al. Rad50 mediates DNA demethylation to establish pluripotent reprogramming. Exp Mol Med 52:1116-1127 (2020). PubMed: 32665583