Western blot abreview for Anti-Rad50 antibody [13B3/2C6]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 160 kDa Non-specific: 300 kDa
Positive control
50 ug HEK293 total cell lysate
Negative control
none

Dr. Philippe Szankasi

Verified customer

Submitted Feb 24 2006

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