Western blot abreview for Anti-Rad50 antibody [13B3/2C6]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (293T and MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T and MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk TBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Cell Signalling Anti-mouse IgG, HRP-linked Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140 kDa Non-specific: none kDa
Positive control
293T

Additional data

Additional Notes
Great antibody, though very weak in mouse fibroblasts.
Abcam response
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Submitted Oct 22 2018

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