Product nameAnti-Rad50 antibody
See all Rad50 primary antibodies
DescriptionRabbit polyclonal to Rad50
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) - which represented a portion of human Rad 50 encoded in partby exons 2 and 3.
- HeLa cell nuclear extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab3622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 157 kDa (predicted molecular weight: 150 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Tissue specificityExpressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Involvement in diseaseDefects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
Sequence similaritiesBelongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain.
DomainThe zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
- Information by UniProt
- DNA repair protein RAD50 antibody
- hRAD50 antibody
- hsRAD50 antibody
1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)
2. IP from 1mg extract with 1.25
µg Rabbit anti-Rad 50 (exon 13-14) (ab3623)
Sample: Nuclear extract from Hela cells
for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution
This product has been referenced in:
- Goldstein M et al. Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. Proc Natl Acad Sci U S A 110:16874-9 (2013). IP, ChIP . Read more (PubMed: 24082117) »
- Tommiska J et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 118:2911-6 (2006). Read more (PubMed: 16385572) »