Asynchronous cells were treated with bleomycin to induce DNA double strand breaks. Cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected nuclear-enriched staining pattern and produces the larger DNA damage associated breaks. MeOH fixed samples were also evaluated and produced a similar, albeit slightly weaker staining pattern.
Dr. Kirk Mcmanus
Submitted Nov 19 2012
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