Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad50 antibody [EPR3466(2)]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were treated with bleomycin to induce DNA double strand breaks. Cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected nuclear-enriched staining pattern and produces the larger DNA damage associated breaks. MeOH fixed samples were also evaluated and produced a similar, albeit slightly weaker staining pattern.

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 19 2012

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