Western blot abreview for Anti-Rad50 antibody [EPR3466(2)]

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Treatment
10 Gy IR
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% BSA
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-Rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 150 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Mock IR
Positive control
+ 10 Gy IR

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Submitted Apr 25 2014

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