Western blot abreview for Anti-Rad50 antibody [EPR3466(2)]

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (MEFs, U2OS, HEK 293 and Fibroblasts)
Specification
MEFs, U2OS, HEK 293 and Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG-peroxidase antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Bands
Specific: 150 kDa Non-specific: 65 kDa
Exposure
4 minute(s) and 0 second(s)

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Submitted Nov 07 2014

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