Overview

  • Product name

    Anti-Rad50 antibody - N-terminal
    See all Rad50 primary antibodies
  • Description

    Rabbit polyclonal to Rad50 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Recombinant fragment within Human Rad50 (N terminal). The exact sequence is proprietary.
    Database link: Q92878

  • Positive control

    • WB: HeLa whole cell and nuclear extracts. Neuro-2a, NT2/D1, PC-12 and GL261 whole cell lysate. IHC-P: Human lung carcinoma tissue. IP: NT2/D1 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 154 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Tissue specificity

    Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • Involvement in disease

    Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • Sequence similarities

    Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • Domain

    The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

Images

  • All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/4000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : Rad50 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 154 kDa



    5% SDS-PAGE

  • Paraffin-embedded human lung carcinoma tissue stained for Rad50 with ab228935 at a 1/250 dilution in immunohistochemical analysis.

  • Rad50 was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate with 4 µg ab228935. Western blot was performed from the immunoprecipitate using ab228935 at a 1/500 dilution.

    Lane 1: 50 µg NT2/D1 whole cell lysate.

    Lane 2: Control with 4 µg preimmune Rabbit IgG.

    Lane 3: ab228935 in NT2/D1 whole cell lysate.

    5% SDS-PAGE

  • All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 154 kDa



    5% SDS-PAGE

  • All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : GL261 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 154 kDa



    5% SDS-PAGE

  • Anti-Rad50 antibody - N-terminal (ab228935) at 1/5000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 154 kDa



    5% SDS-PAGE

  • Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 154 kDa



    5% SDS-PAGE

References

ab228935 has not yet been referenced specifically in any publications.

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