- Datasheet
- Specific References (56)
- Protocols
Overview
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Product nameAnti-Rad51 antibody [14B4]
See all Rad51 primary antibodies -
DescriptionMouse monoclonal [14B4] to Rad51
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Host speciesMouse
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SpecificitySpecific for human Rad51.
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Tested applicationsSuitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein corresponding to Human Rad51 aa 1-338.
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Positive control
- T24 Bladder carcinoma cells and recombinant fusion protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferConstituents: PBS, pH 7.4
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone number14B4
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MyelomaNS1
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IsotypeIgG2b
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. | |
| IP | Use at an assay dependent concentration. |
Application notes
Is unsuitable for ICC/IF.
Target
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FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
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Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
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Involvement in diseaseBreast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
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Post-translational
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
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Database links
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
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Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Images
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![Western blot - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.com/ps/products/0/ab213/Images/ab213-275402-ab213WB.jpg)
Western blot - Anti-Rad51 antibody [14B4] (ab213)Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + Jurkat whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 37 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.com/ps/products/0/ab213/Images/ab213-216007-ab213ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of BT483 xenograft labeling RAD51 with ab213 at 1/200.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.com/ps/products/0/ab213/Images/ab213-200211-ab213ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)This image is courtesy of an anonymous Abreviewab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
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![Western blot - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.com/ps/products/0/ab213/Images/Rad51-Primary-antibodies-ab213-6.jpg)
Western blot - Anti-Rad51 antibody [14B4] (ab213)Image courtesy of an anonymous Abreview.Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg
Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 42,45 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
References
This product has been referenced in:
- Roy S et al. p53 orchestrates DNA replication restart homeostasis by suppressing mutagenic RAD52 and POL? pathways. Elife 7:N/A (2018). Read more (PubMed: 29334356) »
- Bleuyard JY et al. MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress. Proc Natl Acad Sci U S A 114:7671-7676 (2017). WB . Read more (PubMed: 28673974) »
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