Anti-Rad51 antibody [14B4] (ab213)

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for your email.

I would be happy to process refunds for ab213 and ab68715. Could you however let me know why the customer expecting a 170kDa band for ab58101 which should be nearly 130-140 kDa.

Thanks!

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- Could you provide us the images? we may help you with the troubleshooting.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Thank you for contacting Abcam.

I just had a few more protocol questions for you regarding the problems you are having with ab213 in western blot.

1 - Why was 2 different lysis buffers used in the extraction protocol? Either of them should have been suffcient on their own.

2 - Whenyou say that the wetern blotting did not work. Does that means there was no signal, high background, weak signal?

3 - Could you please provide the lot number of the antibody you are using?

I look forward to your reply and helping resolve this situation.

I am happy to help. Best of luck in your experiments!

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab63801.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for contacting us. I am sorry to hear that the antibody is giving problems. As we discussed yesterday over the phone, the following suggestions might help to get rid of the red blob you are seeing in the nuclei.: 1) use less antibody (for the secondary: e.g. 1/500 or even 1/1000, maybe also for the primary: e.g. 1/1000) 2) include a separate blocking step (e.g. 3-5% BSA or 10% goat serum) 3) omit the pre-extraction step Please let me know if these if these suggestions are of help or not. If the tips do not help, please let me know your PO or order number. If the product was purchased within the last 6 months, we can discuss a free of charge replacement, credit or refund according to our Abpromise guarantee. I wish you good luck with your experiments and look forward to hear back from you.