Overview

  • Product name

    Anti-Rad51 antibody [51RAD01]
    See all Rad51 primary antibodies
  • Description

    Mouse monoclonal [51RAD01] to Rad51
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    BALB/C mice were injected with recombinant Rad51 protein.

  • Positive control

    • Testis

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    51RAD01
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/10. PMID 18663141
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
    • Tissue specificity

      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
    • Involvement in disease

      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
    • Sequence similarities

      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.
    • Domain

      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
    • Post-translational
      modifications

      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Information by UniProt
    • Database links

    • Alternative names

      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab1837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1837, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-mouse DyLight® 488 goat (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunofluorescence analysis of wild-type (+/+) and Hormad1-/- (-/-) murine (mouse) zygotene spermatocytes, staining Rad51 with ab1837.

    References

    This product has been referenced in:

    • Bodo S  et al. Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury. J Clin Invest 129:786-801 (2019). Read more (PubMed: 30480549) »
    • Ho TT  et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature 543:205-210 (2017). Read more (PubMed: 28241143) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    21-28 of 28 Abreviews or Q&A

    Answer

    Thank you for contacting us. I am sorry that we were not able to improve your results to your satisfaction. I have asked our accounting department to issue you a full refund. I hope that this experience will not prevent you from purchasing a product from us in the future as we have many quality products. If you have any further questions concerning your refund, please contact the accounting department at accounts@abcam.com. The technical team is always at your service, should you require further expert advice. Best of luck with your research.

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    Answer

    I'm sorry to hear you are having a problem with ab1837. You are using the positive control so you should see a signal. I would like to suggest the following modifications to your protocol: 1) I am concerned that the instructions for using the antibody explicitly state "This antibody may be diluted to a titer of 1:25-1:50 in an ABC method". I would recommend using a biotin conjugated secondary and the ABC method. 2) Are you using paraffin embedded tissue? If not, then you do not need to perform antigen retrieval. 3) If you are using cells, you can fix in acetone and then do not need a permeablization step. 4) I would also recommend trying to block in 10% normal goat serum for 30 min at RT, incubate in primary in PBS for 30 min at RT, and secondary also for 30 min at RT. Please let me know if this helps and do not hesitate to contact us for further advice.

    Read More

    Question
    Answer

    The concentration is 100 ug/ml. Please contact us again if you have any additional questions.

    Read More

    Question
    Answer

    Thank you for your enquiry. Ab1837 has been tested for cross-reactivity with rat and mouse, but has not yet been tested for cross-reactivity with other species (including human). It has been tested for appliaction in Western blot, Immunoprecipitation, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), and Immunofluorescence. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

    Read More

    Answer

    My apologies for the delay in the response. I am please to forward the following information to answer your questions: Ab 213: The immunogen for this particular antibody was full length human Rad51 expressed in and purified from E. coli. The immunogen was Rad51, not one of the Rad51-like isoforms. The exact epitope on hRad51 has not been mapped. Ab1827: The immunogen used was the human Rad51 protein. Ab15849: The immunogen was purified recombinant human Rad51. It is different from any of the paralogues (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3). See Mason J-Y et al.Genes Dev. 2001 Dec 15;15(24):3296-307. I hope this information helps, please do not hesitate to contact us if you need more information,

    Read More

    Question
    Answer

    Thank you for your enquiry. For ab1837 the immunogen was recombinant Rad51 protein. For ab12448, the immunogen that was used was the full length protein, not a peptide fragment. As such, the epitope which this antibody maps to in not currently known. If you have any more questions please contact us again.

    Read More

    Answer

    All the information that we have regarding this antibody is located on the online datasheet. Antibody titer range is given on the datasheet and the most common fixative for IF is cold acetone at 4 C.

    Read More

    Answer

    The specificity of this anti-Rad51 antibody (Clone 51RAD01) with regards to the various homologs has not yet been determined to our knowledge. The customer may want to review the following study for further details: Barlow A.L. et. al. EMBO J. 16 (17):5207-15. Ab 2180 - Clone 2H11/6 that is specific for Rad51C and Ab3637 - Clone 1 H3/13 that is specific for Rad51B. Please refer your customer to our catalogue. We also found the following links to publications in which several anti-Rad51 antibodies were used that might be able to assist the customer in finding a suitable antibody. http://www.jbc.org/cgi/content/full/277/10/8406 http://www.pnas.org/cgi/reprint/98/15/8440.pdf

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    21-28 of 28 Abreviews or Q&A

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