- Datasheet
- Specific References (12)
- Protocols
Overview
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Product nameAnti-Rad51 antibody [51RAD01]
See all Rad51 primary antibodies -
DescriptionMouse monoclonal [51RAD01] to Rad51
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
BALB/C mice were injected with recombinant Rad51 protein.
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Positive control
- Testis
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone number51RAD01
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab1837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | 1/10. PMID 18663141 |
Target
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FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
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Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
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Involvement in diseaseBreast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
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Post-translational
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
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Database links
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Entrez Gene: 499870 Rat
- Omim: 179617 Human
- SwissProt: Q06609 Human
- SwissProt: Q08297 Mouse
- Unigene: 631709 Human
- Unigene: 330492 Mouse
see all -
Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Images
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![Flow Cytometry - Anti-Rad51 antibody [51RAD01] (ab1837)](https://www.abcam.com/ps/products/1/ab1837/Images/Rad51-Primary-antibodies-ab1837-1.jpg)
Flow Cytometry - Anti-Rad51 antibody [51RAD01] (ab1837)Overlay histogram showing HeLa cells stained with ab1837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1837, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-mouse DyLight® 488 goat (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [51RAD01] (ab1837)](https://www.abcam.com/ps/products/1/ab1837/Images/Rad51-Primary-antibodies-ab1837-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [51RAD01] (ab1837)Image from Shin YH et al., PLoS Genet. 2010 Nov 4;6(11):e1001190.Fig 5.; doi:10.1371/journal.pgen.1001190November 4, 2010, PLoS Genet 6(11): e1001190.Immunofluorescence analysis of wild-type (+/+) and Hormad1-/- (-/-) murine zygotene spermatocytes, staining Rad51 with ab1837.
Protocols
References
This product has been referenced in:
- Venkatachalam G et al. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res 45:10564-10582 (2017). Read more (PubMed: 28985345) »
- Carofiglio F et al. SPO11-independent DNA repair foci and their role in meiotic silencing. PLoS Genet 9:e1003538 (2013). ICC/IF . Read more (PubMed: 23754961) »
Customer reviews and Q&As
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