Western blot abreview for Anti-Rad51 antibody [51RAD01]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
10 µg
Specification
Hela
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS BSA 1%
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 15 kDa

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Submitted Oct 21 2017

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