Western blot abreview for Anti-Rad51 antibody [51RAD01]

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Melanoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 100°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
N/A
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 27 kDa
Positive control
Non-treated cell

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Submitted Apr 19 2019

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