Key features and details
- Rabbit polyclonal to Rad51
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Rad51 antibody
See all Rad51 primary antibodies
DescriptionRabbit polyclonal to Rad51
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 1-228 of Human Rad51.
- Recombinant human Rad51 protein (ab63808) can be used as a positive control in WB. H1299, HepG2, NIH-3T3 whole cell lysates; 293T, A431, MOLT4, Raji cell lines; H1299, HeLa cells; Cal27 Human xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab137323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.Can be blocked with Recombinant human Rad51 protein (Active) (ab63808).|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.|
FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Involvement in diseaseBreast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
Anti-Rad51 antibody (ab137323) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS Page
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Cal27 Human xenograft tissue labelling Rad51, using ab137323 at a 1/100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells staining Rad51, using ab137323 (green) at a 1/200 dilution. Cell nuclei are shown in blue.
All lanes : Anti-Rad51 antibody (ab137323) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
12% SDS Page
Immunofluorescence analysis of paraformaldehyde-fixed H1299, labelling Rad51 using ab137323 at 1/400 dilution. Middle panel cell nuclei are shown in blue. Lower panel merged.
ab137323 has been referenced in 4 publications.
- Maidarti M et al. Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response. Hum Reprod N/A:N/A (2018). PubMed: 30521029
- Dréan A et al. Modeling Therapy Resistance in BRCA1/2-Mutant Cancers. Mol Cancer Ther 16:2022-2034 (2017). PubMed: 28619759
- Botthof JG et al. Loss of the homologous recombination generad51leads to Fanconi anemia-like symptoms in zebrafish. Proc Natl Acad Sci U S A 114:E4452-E4461 (2017). WB . PubMed: 28512217
- Markiewicz E et al. Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape. Open Biol 5:150011 (2015). PubMed: 25924630