Overview

  • Product name

  • Description

    Rabbit polyclonal to Rad51
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, ChIPmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant Rad51 protein (Saccharomyces cerevisiae) purified.

  • Positive control

    • Extract of wild-type S.cervisiae strain.

Properties

Applications

Our Abpromise guarantee covers the use of ab63798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration. PubMed: 22343724

Target

  • Function

    Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificity

    Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in disease

    Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similarities

    Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • Domain

    The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • Alternative names

    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • FANCR antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Images

  • Anti-Rad51 antibody (ab63798) at 1/1000 dilution + S, cerevisiae crude extract

    Secondary
    HRP conjugated goat anti-rabbit IgG antibody at 1/10000 dilution

    Predicted band size: 43 kDa

References

This product has been referenced in:

  • Pal S  et al. Impaired cohesion and homologous recombination during replicative aging in budding yeast. Sci Adv 4:eaaq0236 (2018). Read more (PubMed: 29441364) »
  • Ma E  et al. Rad52-Rad51 association is essential to protect Rad51 filaments against Srs2, but facultative for filament formation. Elife 7:N/A (2018). Read more (PubMed: 29985128) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test ab63798 for ChIP. This code will give you 1 free primary antibody before the expiration date. Your move from one institute to another should not have an effect in this. As long as you keep a note of the testing discount code i issue and the original order number this should not be a problem. This is your discount code and expiry date: DISCOUNT CODE: xxxxxx Expiration date: xxxx-xx-xx To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. The Anti-Rad51 antibody (ab63798) you are interested in has to our knowledge not been tested with ChIP as of yet. However, if you choose to purchase this antibody you would qualify for a testing discount, more information of which can be found in the link below: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount The discount must be issued before you purchase the antibody so please, if you are interested in this offer email to confirm before purchase. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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