Anti-Rad51 antibody (ab63801)
Key features and details
- Rabbit polyclonal to Rad51
- Suitable for: WB, ICC/IF
- Reacts with: Human, Xenopus laevis
- Isotype: IgG
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Overview
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Product name
Anti-Rad51 antibody
See all Rad51 primary antibodies -
Description
Rabbit polyclonal to Rad51 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Xenopus laevis -
Immunogen
Purified recombinant full length protein (Human)
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Positive control
- WB: Crude extract of Xenopus; HeLa cell extract. ICC: U2OS cells.
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General notes
Please click here for Instructions For Use/Protocol.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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ICC/IF | (5) |
1/100.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
1/100. |
Target
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Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
Tissue specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
Involvement in disease
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
Domain
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
Post-translational
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
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Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Images
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2BN hTert (XLF-deficient) human fibroblasts were fixed for 30 min with methanol at −20°C, dipped for 1 min in ice cold acetone for permeabilization and washed three times for 10 min with PBS/1% FCS. Non-specific antigens were blocked for 30 min in 5% BSA in PBS/1% FCS. Samples were incubated with ab63801 (1∶15000) in PBS/1% FCS over night at 4°C, washed three times in PBS/1% FCS and incubated for 1 h at room temperature with AlexaFluor 488 conjugated secondary antibodies (1 : 500). After three times of washing in PBS, cells were DAPI stained.
Image shows 2BN hTert (XLF-deficient) human fibroblasts analyzed 2 h post IR with 1 Gy.
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Anti-Rad51 antibody (ab63801) at 1/1000 dilution + Crude extract of Xenopus
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma U2OS cells X-ray irradiation before and 2h after, immunostained with Anti-Rad51 antibody (ab63801) at 1/6,000 dilution. The lower panels were the same cells with Hoechst.
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All lanes : Anti-Rad51 antibody (ab63801) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : HeLa cell extract
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801 (x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (114)
ab63801 has been referenced in 114 publications.
- Hariharasudhan G et al. TOPORS-mediated RAD51 SUMOylation facilitates homologous recombination repair. Nucleic Acids Res 50:1501-1516 (2022). PubMed: 35061896
- Shih DJH et al. Exploiting induced vulnerability to overcome PARPi resistance and clonal heterogeneity in BRCA mutant triple-negative inflammatory breast cancer. Am J Cancer Res 12:337-354 (2022). PubMed: 35141022
- Kolinjivadi AM et al. The H. pylori CagA Oncoprotein Induces DNA Double Strand Breaks through Fanconi Anemia Pathway Downregulation and Replication Fork Collapse. Int J Mol Sci 23:N/A (2022). PubMed: 35163588
- Xin Y et al. Identification of Nanog as a novel inhibitor of Rad51. Cell Death Dis 13:193 (2022). PubMed: 35220392
- Ma H et al. HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer. Cell Death Dis 13:263 (2022). PubMed: 35332131