Key features and details
- Chicken polyclonal to Rad51
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgY
Product nameAnti-Rad51 antibody
See all Rad51 primary antibodies
DescriptionChicken polyclonal to Rad51
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Purified recombinant full length protein (Human)
- WB: HeLa cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab63802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/7000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Involvement in diseaseBreast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
All lanes : Anti-Rad51 antibody (ab63802) at 1/2000 dilution
Lane 1 : Molecular weight marker
Lane 2 : HeLa cell extract
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells, staining Rad51 with ab63802.
Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in 1X PBS and blocked with 5% BSA for 15 mins at 20°C. Samples were incubated with primary antibody (1/500 in 5% BSA in PBS) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-chicken polyclonal IgG was used as the secondary antibody.
ab63802 has not yet been referenced specifically in any publications.