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    rad51-antibody-epr40303-ab133534.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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RecombinantRabMAb

Recombinant Anti-Rad51 antibody [EPR4030(3)] (ab133534)

  • Datasheet
  • SDS
Reviews (13)Q&A (1)References (45)

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Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Immunoprecipitation - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • OI-RD Scanning - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
  • Anti-Rad51 antibody [EPR4030(3)] (ab133534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4030(3)] to Rad51
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Primary
Product image
Alexa Fluor® 488 Anti-Rad51 antibody [EPR4030(3)] (ab196449)
Protein
Product image
Recombinant human Rad51 protein (Active) (ab63808)

View more associated products

Overview

  • Product name

    Anti-Rad51 antibody [EPR4030(3)]
    See all Rad51 primary antibodies
  • Description

    Rabbit monoclonal [EPR4030(3)] to Rad51
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Rad51 aa 1-100. The exact sequence is proprietary.
    Database link: Q06609
    (Peptide available as ab176353)

  • Positive control

    • WB: C6, NIH/3T3, 293T, Jurkat, HeLa, and K562 cell lysates and mouse spleen tissue lysate. IHC-P: Human cervix carcinoma and testis tissues. ICC/IF: Jurkat cells. Flow Cyt: HeLa cells. IP: HEK293 cell lysates.
  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4030(3)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Alternative Versions

    • HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)
    • Alexa Fluor® 488 Anti-Rad51 antibody [EPR4030(3)] (ab196449)
    • Anti-Rad51 antibody [EPR4030(3)] - BSA and Azide free (ab221796)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Mouse spleen tissue lysate - total protein (14 days) (ab7274)
    • Mouse spleen tissue lysate - total protein (7 days) (ab7275)
    • Jurkat whole cell lysate (ab7899)
    • HEK-293 whole cell lysate (ab7902)
    • K-562 whole cell lysate (ab7911)
    • Mouse small intestine tissue lysate - total protein (ab7939)
  • Recombinant Protein

    • Recombinant human Rad51 protein (Active) (ab63808)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Rat
Human
Application Abreviews Notes
WB (6)
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P (2)
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (4)
1/1000.

For unpurified use at 1/100 - 1/250.

Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/10 - 1/100.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/1000.

For unpurified use at 1/100 - 1/250.

Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/10 - 1/100.

Target

  • Function

    Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificity

    Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in disease

    Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similarities

    Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • Domain

    The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Target information above from: UniProt accession Q06609 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5888 Human
    • Entrez Gene: 19361 Mouse
    • Entrez Gene: 499870 Rat
    • Omim: 179617 Human
    • SwissProt: Q06609 Human
    • SwissProt: Q08297 Mouse
    • Unigene: 631709 Human
    • Unigene: 330492 Mouse
    • Unigene: 20474 Rat
    see all
  • Alternative names

    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • FANCR antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling Rad51 with purified ab133534 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/1000) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    All lanes : Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution (purified)

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling Rad51 with purified ab133534 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Rad51 with purified ab133534 at 1/350 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Immunoprecipitation - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    ab133534 (purified) at 1/100 immunoprecipitating Rad51 in HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate.

    Lane 1 (input): HEK-293 whole cell lysate (10µg)

    Lane 2 (+): ab133534 + HEK-293 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133534 in HEK-293 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [EPR4030(3)] (ab133534)Gao et al Nat Commun. 2018; 9: 3925. Published online 2018 Sep 25. doi: 10.1038/s41467-018-06407-7

    Immunocytochemical analysis of DT40 cells (WT and the indicated KOs) labeling Rad51 using ab133534 at 1/200 dilution.

    From Figure 5d of gao et al.

    Gao et al Nat Commun. 2018; 9: 3925. Published online 2018 Sep 25. doi: 10.1038/s41467-018-06407-7

    Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/.

  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution (purified) + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    All lanes : Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/20000 dilution (purified)

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Western blot - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    All lanes : Anti-Rad51 antibody [EPR4030(3)] (ab133534) at 1/10000 dilution (unpurified)

    Lane 1 : C6 (rat glial tumor cell line) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Rad51 with unpurified ab133534 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Flow Cytometry - Anti-Rad51 antibody [EPR4030(3)] (ab133534)

    Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab133534 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab133534, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • OI-RD Scanning - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    OI-RD Scanning - Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Rad51 antibody [EPR4030(3)] (ab133534)
    Anti-Rad51 antibody [EPR4030(3)] (ab133534)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (45)

    Publishing research using ab133534? Please let us know so that we can cite the reference in this datasheet.

    ab133534 has been referenced in 45 publications.

    • Sun C  et al. MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC. J Hematol Oncol 13:9 (2020). PubMed: 32005272
    • Dongol S  et al. IQGAP3 promotes cancer proliferation and metastasis in high-grade serous ovarian cancer. Oncol Lett 20:1179-1192 (2020). PubMed: 32724358
    • Zhou Q  et al. The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair. Nat Commun 11:2639 (2020). PubMed: 32457312
    • Tang X  et al. The PI3K/mTOR dual inhibitor BEZ235 nanoparticles improve radiosensitization of hepatoma cells through apoptosis and regulation DNA repair pathway. Nanoscale Res Lett 15:63 (2020). PubMed: 32219609
    • Fu E  et al. M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB-EGF. Oncol Rep 44:698-710 (2020). PubMed: 32627036
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 14 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 29 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 29 2019

    Western blot abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 04 2019

    Western blot abreview for Anti-Rad51 antibody [EPR4030(3)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Mouse Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C42B Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jul 08 2019

    Western blot abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jun 26 2019

    Western blot abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    15 µg
    Specification
    Melanoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [EPR4030(3)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Glioblastoma cells U87)
    Permeabilization
    Yes - PBST 0.3%
    Specification
    Glioblastoma cells U87
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Matthias Dedobbeleer

    Verified customer

    Submitted Jun 01 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rad51 antibody [EPR4030(3)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (Bovine ovaries (cortex))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    No
    Specification
    Bovine ovaries (cortex)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rad51 antibody [EPR4030(3)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (testis)
    Specification
    testis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Henry King

    Verified customer

    Submitted Dec 24 2013

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