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  1. Link

    rad51-antibody-rad512702-bsa-and-azide-free-ab269687.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-Rad51 antibody [RAD51/2702] - BSA and Azide free (ab269687)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269687)
  • Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269687)
  • Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269687)

Key features and details

  • Mouse monoclonal [RAD51/2702] to Rad51 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, Protein Array
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Rad51 antibody [RAD51/2702] - BSA and Azide free
    See all Rad51 primary antibodies
  • Description

    Mouse monoclonal [RAD51/2702] to Rad51 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Rad51 aa 1-134. The exact sequence is proprietary.
    Database link: Q06609

  • Positive control

    • Flow cyt: K562 cells. IHC-P: Human colon carcinoma tissue.
  • General notes

    ab269687 is the carrier-free version of ab269677.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    RAD51/2702
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Alternative Versions

    • Anti-Rad51 antibody [RAD51/2702] (ab269677)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Related Products

    • Recombinant human Rad51 protein (Active) (ab63808)
    • Recombinant Human Rad51 protein (ab81943)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 - 2 µg/ml.
Protein Array
Use at an assay dependent concentration.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 - 2 µg/ml.
Protein Array
Use at an assay dependent concentration.

Target

  • Function

    Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificity

    Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in disease

    Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similarities

    Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • Domain

    The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Target information above from: UniProt accession Q06609 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5888 Human
    • Omim: 179617 Human
    • SwissProt: Q06609 Human
    • Unigene: 631709 Human
    • Alternative names

      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269687)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [RAD51/2702] (ab269687)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Rad51 using ab269677 at 2 μg/ml in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium Azide (ab269677).

    • Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269687)
      Flow Cytometry - Anti-Rad51 antibody [RAD51/2702] (ab269687)

      Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Rad51 using ab269677 ar 2 μg/ml (Blue) compared to an isotype control (Red). The secondary antibody is a Goat anti-Mouse IgG-CF488 conjugate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium Azide (ab269677).

    • Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269687)
      Protein Array - Anti-Rad51 antibody [RAD51/2702] (ab269687)

      Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269677.

      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

      This data was developed using the same antibody clone in adifferent buffer formulation containinfg PBS, BSA and Sodium Azide (ab269677).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab269687? Please let us know so that we can cite the reference in this datasheet.

    ab269687 has not yet been referenced specifically in any publications.

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