Product nameRad51 overexpression 293T lysate (whole cell)
See all Rad51 lysates
ab94044 is a 293T cell transfected lysate in which Human Rad51 has been transiently over-expressed using a pCMV-Rad51 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundDisease: Breast cancer Mirror movements 2 Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. Domain: The nuclear localization may reside in the C-terminus (between 259 and 339 AA). Function: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. PTM: Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. Similarity: Belongs to the RecA family. RAD51 subfamily. Contains 1 HhH domain. Tissue specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Our Abpromise guarantee covers the use of ab94044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94044 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Rad51 antibody (ab88572) at 1/500 dilution
Lane 1 : ab94044
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94044 has not yet been referenced specifically in any publications.