Overview

  • Product name

  • Description

    Rabbit polyclonal to RAD51AP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human RAD51AP1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115144)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; U2OS; Raji.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/200. Fix cells using paraformaldegyde and permeabilize using 0.5% Triton X-100/PBS.

Target

  • Function

    May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.
  • Tissue specificity

    Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Colocalizes with RAD51 to multiple nuclear foci.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2510006L10Rik antibody
    • MGC156698 antibody
    • OTTMUSP00000028651 antibody
    • PIR51 antibody
    • R51A1_HUMAN antibody
    • RAB22 antibody
    • RAD51 associated protein 1 antibody
    • RAD51 interacting protein antibody
    • RAD51-associated protein 1 antibody
    • RAD51-interacting protein antibody
    • Rad51ap1 antibody
    see all

Images

  • All lanes : Anti-RAD51AP1 antibody (ab101321) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes
  • ab101321 (1/200) staining RAD51AP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References

This product has been referenced in:

  • Wu Y  et al. Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thorac Cancer 10:1748-1763 (2019). Read more (PubMed: 31317661) »
  • Cukras S  et al. The USP1-UAF1 complex interacts with RAD51AP1 to promote homologous recombination repair. Cell Cycle 15:2636-2646 (2016). Read more (PubMed: 27463890) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 17 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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