This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; U2OS; Raji.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
1/200. Fix cells using paraformaldegyde and permeabilize using 0.5% Triton X-100/PBS.
May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.
Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Colocalizes with RAD51 to multiple nuclear foci.
All lanes : Anti-RAD51AP1 antibody (ab101321) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa Observed band size: 38 kDa Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Immunocytochemistry/ Immunofluorescence - Anti-RAD51AP1 antibody (ab101321)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab101321 (1/200) staining RAD51AP1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.