Anti-RAD51AP1 antibody (ab88370)
Key features and details
- Mouse polyclonal to RAD51AP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAD51AP1 antibody -
Description
Mouse polyclonal to RAD51AP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RAD51AP1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human colon tissue lysate, RAD51AP1 transfected 293T cell lysate, HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA. -
Tissue specificity
Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Colocalizes with RAD51 to multiple nuclear foci. - Information by UniProt
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Database links
- Entrez Gene: 10635 Human
- Omim: 603070 Human
- SwissProt: Q96B01 Human
- Unigene: 504550 Human
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Alternative names
- 2510006L10Rik antibody
- MGC156698 antibody
- OTTMUSP00000028651 antibody
see all
Images
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Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml + Human colon tissue lysate at 50 µg
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml
Lane 1 : RAD51AP1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa -
ab88370 at 10µg/ml staining RAD51AP1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88370 has been referenced in 2 publications.
- Lee CY et al. Promotion of homology-directed DNA repair by polyamines. Nat Commun 10:65 (2019). PubMed: 30622262
- Liang F et al. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nat Commun 10:2849 (2019). PubMed: 31253762