Overview

  • Product name
  • Description
    Mouse polyclonal to RAD51AP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human RAD51AP1, amino acids 1-335 (NP_006470.1).

  • Positive control
    • Human colon tissue lysate, RAD51AP1 transfected 293T cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.
  • Tissue specificity
    Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Colocalizes with RAD51 to multiple nuclear foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2510006L10Rik antibody
    • MGC156698 antibody
    • OTTMUSP00000028651 antibody
    • PIR51 antibody
    • R51A1_HUMAN antibody
    • RAB22 antibody
    • RAD51 associated protein 1 antibody
    • RAD51 interacting protein antibody
    • RAD51-associated protein 1 antibody
    • RAD51-interacting protein antibody
    • Rad51ap1 antibody
    see all

Images

  • Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size: 37 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml

    Lane 1 : RAD51AP1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • ab88370 at 10µg/ml staining RAD51AP1 in HeLa cells by Immunofluorescence.

References

ab88370 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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