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    rad52-antibody-epr34642-ab124971.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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RecombinantRabMAb

Recombinant Anti-RAD52 antibody [EPR3464(2)] (ab124971)

  • Datasheet
  • SDS
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Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
  • Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
  • OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
  • Anti-RAD52 antibody [EPR3464(2)] (ab124971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3464(2)] to RAD52
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RAD52 antibody [EPR3464(2)]
    See all RAD52 primary antibodies
  • Description

    Rabbit monoclonal [EPR3464(2)] to RAD52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RAD52 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK-293T and PC3 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 3.00 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3464(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Alternative Versions

    • Anti-RAD52 antibody [EPR3464(2)] - BSA and Azide free (ab240010)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant Human RAD52 protein (ab188614)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).

Target

  • Function

    Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • Sequence similarities

    Belongs to the RAD52 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P43351 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5893 Human
    • Omim: 600392 Human
    • SwissProt: P43351 Human
    • Unigene: 410355 Human
    • Alternative names

      • DNA repair protein RAD52 antibody
      • DNA repair protein RAD52 antibody
      • DNA repair protein RAD52 homolog antibody
      • RAD 52 antibody
      • Rad52 antibody
      • RAD52 homolog (S. cerevisiae) antibody
      • RAD52 homolog (S. cerevisiae) antibody
      • RAD52 homolog antibody
      • RAD52_HUMAN antibody
      • Recombination protein RAD52 antibody
      • Recombination protein RAD52 antibody
      • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
      see all

    Images

    • Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      Lane 1 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution
      Lane 2 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 46 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 46 kDa

    • OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-RAD52 antibody [EPR3464(2)] (ab124971)
      Anti-RAD52 antibody [EPR3464(2)] (ab124971)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab124971? Please let us know so that we can cite the reference in this datasheet.

    ab124971 has been referenced in 3 publications.

    • Gong H  et al. p73 coordinates with ?133p53 to promote DNA double-strand break repair. Cell Death Differ N/A:N/A (2018). PubMed: 29511339
    • Tong L  et al. RDM1 plays an oncogenic role in human lung adenocarcinoma cells. Sci Rep 8:11525 (2018). PubMed: 30069034
    • Gong L  et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). PubMed: 27874035

    Customer reviews and Q&As

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