Anti-RAD52 antibody [EPR3464(2)] (ab124971)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-RAD52 antibody [EPR3464(2)]
See all RAD52 primary antibodies -
DescriptionRabbit monoclonal [EPR3464(2)] to RAD52
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human RAD52 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, 293T, and PC3 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 3.00 x 10 -12 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3464(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). |
Target
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FunctionInvolved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
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Sequence similaritiesBelongs to the RAD52 family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5893 Human
- Omim: 600392 Human
- SwissProt: P43351 Human
- Unigene: 410355 Human
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Alternative names
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 homolog antibody
see all
Images
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![Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/RAD52-Primary-antibodies-ab124971-1.jpg)
Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa -
![Other - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/RAD52-Primary-antibodies-ab124971-2.jpg)
Other - Anti-RAD52 antibody [EPR3464(2)] (ab124971)
Protocols
References
This product has been referenced in:
- Gong H et al. p73 coordinates with ?133p53 to promote DNA double-strand break repair. Cell Death Differ N/A:N/A (2018). Read more (PubMed: 29511339) »
- Gong L et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). Read more (PubMed: 27874035) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"