Recombinant Anti-RAD52 antibody [EPR3464(2)] (ab124971)
![Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/ab124971-351366-anti-rad52-antibody-epr3464-2-westernblot-human.jpg "Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3464(2)] to RAD52
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-RAD52 antibody [EPR3464(2)]
See all RAD52 primary antibodies -
Description
Rabbit monoclonal [EPR3464(2)] to RAD52 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RAD52 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK-293T and PC3 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 3.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3464(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). |
Target
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Function
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase. -
Sequence similarities
Belongs to the RAD52 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5893 Human
- Omim: 600392 Human
- SwissProt: P43351 Human
- Unigene: 410355 Human
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Alternative names
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 antibody
- DNA repair protein RAD52 homolog antibody
see all
Images
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Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)Lane 1 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution
Lane 2 : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/ab124971-139059-anti-rad52-antibody-epr34642-western-blot.jpg)
Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971)All lanes : Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 46 kDa -
![OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/ab124971-139058-anti-rad52-antibody-epr34642.jpg)
OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (ab124971) -
![Anti-RAD52 antibody [EPR3464(2)] (ab124971)](https://www.abcam.com/ps/products/124/ab124971/Images/ab124971-4-benefits-of-recombinant-antibodies.png)
Anti-RAD52 antibody [EPR3464(2)] (ab124971)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124971 has been referenced in 3 publications.
- Gong H et al. p73 coordinates with ?133p53 to promote DNA double-strand break repair. Cell Death Differ N/A:N/A (2018). PubMed: 29511339
- Tong L et al. RDM1 plays an oncogenic role in human lung adenocarcinoma cells. Sci Rep 8:11525 (2018). PubMed: 30069034
- Gong L et al. p53 isoform ?133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep 6:37281 (2016). PubMed: 27874035