For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Saccharomyces cerevisiae Rad53.
Our Abpromise guarantee covers the use of ab104232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 25817432|
|WB||1/2000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). Abcam recommends using milk as the blocking agent (4%)|
Blocking: 4% milk for 1 hour
Phosphorylation of Rad53 induced by phleomycin treatment see PubmedID:152856
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"