Anti-Rad53 antibody [EL7.E1] (ab166859)
Key features and details
- Mouse monoclonal [EL7.E1] to Rad53
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-Rad53 antibody [EL7.E1]
See all Rad53 primary antibodies -
Description
Mouse monoclonal [EL7.E1] to Rad53 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae
Does not react with: Mouse, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
EL7.E1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Function
Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.
Contains 2 FHA domains.
Contains 1 protein kinase domain. -
Domain
FHA domains are phosphothreonine recognition modules, FHA 1 strongly selects for Asp at position +3 relative to phosphothreonine, whereas FHA 2 selects for Ile in this position. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 855950 Saccharomyces cerevisiae
- SwissProt: P22216 Saccharomyces cerevisiae
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Alternative names
- CHEK2 homolog antibody
- CHK2 homolog antibody
- MEC2 antibody
see all
Images
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All lanes : Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/ml
Lane 1 : S. Cerevisiae untreated
Lane 2 : S. Cerevisiae MMS treated
Lysates/proteins at 5 µl per lane.
Secondary
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesWestern Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.
Blocking buffer: 3% Milk
Developed using the ECL technique.
Performed under reducing conditions.
Additional bands at: 55 kDa. Identity unknown.
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All lanes : Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/ml
Lane 1 : TCA preps from S. cerevisiae cells
Lane 2 : TCA preps from S. cerevisiae cells treated with Hydroxyurea (HU)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesWestern Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.
Datasheets and documents
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Datasheet download
References (22)
ab166859 has been referenced in 22 publications.
- Kissling VM et al. Mre11-Rad50 oligomerization promotes DNA double-strand break repair. Nat Commun 13:2374 (2022). PubMed: 35501303
- Appanah R et al. Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability. Cell Rep 30:2094-2105.e9 (2020). PubMed: 32075754
- Memisoglu G et al. Mec1ATR Autophosphorylation and Ddc2ATRIP Phosphorylation Regulates DNA Damage Checkpoint Signaling. Cell Rep 28:1090-1102.e3 (2019). PubMed: 31340146
- Biswas H et al. Ddc2ATRIP promotes Mec1ATR activation at RPA-ssDNA tracts. PLoS Genet 15:e1008294 (2019). PubMed: 31369547
- House NC et al. Distinct roles for S. cerevisiae H2A copies in recombination and repeat stability, with a role for H2A.1 threonine 126. Elife 8:N/A (2019). PubMed: 31804179