• Product name

  • Description

    Rabbit polyclonal to RAD54
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RAD54 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90365)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293 as well as HeLa Nuclear lysate in Western blot. This antibody also gave a positive signal in Immunohistochemistry within Human breast adenocarcinoma formalin fixed paraffin embedded tissue section.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab10705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • DNA repair and recombination protein RAD54 like antibody
      • DNA repair and recombination protein RAD54-like antibody
      • hHR 54 antibody
      • hHR54 antibody
      • HR 54 antibody
      • HR54 antibody
      • hRAD 54 antibody
      • hRAD54 antibody
      • RAD 54A antibody
      • RAD 54L antibody
      • RAD54 (S. cerevisiae) like antibody
      • RAD54 homolog antibody
      • RAD54 like (S. cerevisiae) antibody
      • RAD54 like antibody
      • RAD54 like protein antibody
      • RAD54_HUMAN antibody
      • RAD54A antibody
      • RAD54L antibody
      see all


    • Lanes 2-6 : Anti-RAD54 antibody (ab10705) at 1/500 dilution

      Lane 1 : Marker
      Lane 2 : HeLa nuclear cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A432 cell lysate
      Lane 5 : MCF7 cell lysate
      Lane 6 : Hek 293 cell lysate

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

      Predicted band size: 84.3 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?

      Rabbit polyclonal to RAD54 (ab10705) at 1/500.

      Lane 1 Marker
      Lane 2 HeLa nuclear extract
      Lane 3 HeLa cell lysate
      Lane 4 A432 cell lysate
      Lane 5 MCF7 cell lysate
      Lane 6 HEK 293 cell lysate

      Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.

    • RAD54 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RAD54 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10705.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 70kDa; RAD54, non specific band: 75 kDa, We are unsure as to the identity of this extra band. 99kDa band:due to background seen in No ab control lane (2).

    • IHC image of ab10705 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    This product has been referenced in:

    • Tripathi V  et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). Read more (PubMed: 29523790) »
    • Oji Y  et al. Wilms' tumor gene WT1 promotes homologous recombination-mediated DNA damage repair. Mol Carcinog 54:1758-71 (2015). Read more (PubMed: 25418835) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
    Loading amount
    50 µg
    Mouse embryonic fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 25 2007

    This product is known to not work in this application or species.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa and SK-N-SH)
    HeLa and SK-N-SH

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 25 2006

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