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  1. Link

    rad54-antibody-rad54-4e31-ab11055.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)

  • Datasheet
Reviews (4) Submit a question References (6)

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Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)

    Key features and details

    • Mouse monoclonal [Rad54 4E3/1] to RAD54
    • Suitable for: IP, WB, ICC/IF, Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-RAD54 antibody [Rad54 4E3/1]
      See all RAD54 primary antibodies
    • Description

      Mouse monoclonal [Rad54 4E3/1] to RAD54
    • Host species

      Mouse
    • Tested applications

      Suitable for: IP, WB, ICC/IF, Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein corresponding to Human RAD54.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      Rad54 4E3/1
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11055 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP (1)
    Use at an assay dependent concentration.
    WB (2)
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration.
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q92698 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8438 Human
      • Omim: 603615 Human
      • SwissProt: Q92698 Human
      • Unigene: 642042 Human
      • Alternative names

        • DNA repair and recombination protein RAD54 like antibody
        • DNA repair and recombination protein RAD54-like antibody
        • hHR 54 antibody
        • hHR54 antibody
        • HR 54 antibody
        • HR54 antibody
        • hRAD 54 antibody
        • hRAD54 antibody
        • RAD 54A antibody
        • RAD 54L antibody
        • RAD54 (S. cerevisiae) like antibody
        • RAD54 homolog antibody
        • RAD54 like (S. cerevisiae) antibody
        • RAD54 like antibody
        • RAD54 like protein antibody
        • RAD54_HUMAN antibody
        • RAD54A antibody
        • RAD54L antibody
        see all

      Images

      • Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)
        Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)
        Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (6)

      Publishing research using ab11055? Please let us know so that we can cite the reference in this datasheet.

      ab11055 has been referenced in 6 publications.

      • Zhou Q  et al. The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair. Nat Commun 11:2639 (2020). PubMed: 32457312
      • Kondrashova O  et al. Secondary Somatic Mutations RestoringRAD51CandRAD51DAssociated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma. Cancer Discov 7:984-998 (2017). PubMed: 28588062
      • Beck C  et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). ICC/IF ; Human . PubMed: 24598253
      • Mason JM  et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res 74:3546-55 (2014). PubMed: 24753542
      • Muylaert I & Elias P Contributions of nucleotide excision repair, DNA polymerase eta, and homologous recombination to replication of UV-irradiated herpes simplex virus type 1. J Biol Chem 285:13761-8 (2010). WB ; Human . PubMed: 20215648
      • Murzik U  et al. Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell 19:2926-35 (2008). Human . PubMed: 18463164

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-RAD54 antibody [Rad54 4E3/1]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
      Loading amount
      40 µg
      Specification
      Mouse embryonic fibroblasts
      Gel Running Conditions
      Reduced Denaturing (10% Tris-Gly gel)
      Blocking step
      LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 07 2011

      Immunoprecipitation abreview for Anti-RAD54 antibody [Rad54 4E3/1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Total protein in input
      200 µg
      Specification
      HeLa cells
      Immuno-precipitation step
      Protein A/G
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 08 2011

      Western blot abreview for Anti-RAD54 antibody [Rad54 4E3/1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HCT116p53wt cells)
      Loading amount
      30 µg
      Specification
      HCT116p53wt cells
      Gel Running Conditions
      Reduced Denaturing (4-20% Tris-Gly gel)
      Blocking step
      LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 09 2010

      Immunocytochemistry/ Immunofluorescence abreview for Anti-RAD54 antibody [Rad54 4E3/1]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa and SK-N-SH)
      Specification
      HeLa and SK-N-SH
      Fixative
      Paraformaldehyde
      Read More

      DR. Kirk Mcmanus

      Verified customer

      Submitted Aug 25 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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