Anti-ARIP4 antibody (ab86063)
Key features and details
- Rabbit polyclonal to ARIP4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ARIP4 antibody
See all ARIP4 primary antibodies -
Description
Rabbit polyclonal to ARIP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Elephant -
Immunogen
Synthetic peptide corresponding to Human ARIP4 aa 75-125.
Database link: NP_055921.2 -
Positive control
- HeLa whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab86063 was affinity purified using an epitope specific to ARIP4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus. Localizes in speckle-like nuclear compartments. - Information by UniProt
-
Database links
- Entrez Gene: 23132 Human
- Entrez Gene: 81000 Mouse
- Entrez Gene: 363135 Rat
- SwissProt: Q9Y4B4 Human
- SwissProt: Q99NG0 Mouse
- Unigene: 105399 Human
- Unigene: 246689 Mouse
-
Alternative names
- Androgen receptor interacting protein 4 antibody
- Androgen receptor-interacting protein 4 antibody
- Arip4 antibody
see all
Images
-
All lanes : Anti-ARIP4 antibody (ab86063) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 163 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling ARIP4 with ab86063 at 1/1200 (1µg/ml). Detection: DAB.
-
Detection of ARIP4 by Western Blot of Immunprecipitate.
ab86063 at 1µg/ml staining ARIP4 in HeLa whole cell lysate immunoprecipitated using ab86063 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab86063 has been referenced in 1 publication.
- Suzuki J et al. Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proc Natl Acad Sci U S A 113:9509-14 (2016). WB . PubMed: 27503893