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Rabbit polyclonal to RAD54L2
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Elephant
Synthetic peptide, corresponding to a region between residues 75 and 125 of human RAD54L2 (NP_055921.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab86063 was affinity purified using an epitope specific to RAD54L2 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro.
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.
Nucleus. Localizes in speckle-like nuclear compartments.
Information by UniProt
Androgen receptor interacting protein 4 antibody
Androgen receptor-interacting protein 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD54L2 antibody (ab86063)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RAD54L2 with ab86063 at 1/1200 (1µg/ml). Detection: DAB.
Western blot - Anti-RAD54L2 antibody (ab86063)
All lanes :
Anti-RAD54L2 antibody (ab86063) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Predicted band size:
Observed band size:
190 kDa (
why is the actual band size different from the predicted?
Additional bands at:
100 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-RAD54L2 antibody (ab86063)
Detection of RAD54L2 by Western Blot of Immunprecipitate.
ab86063 at 1µg/ml staining RAD54L2 in HeLa whole cell lysate immunoprecipitated using ab86063 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
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