Anti-Rad6 antibody [2782C5] (ab50889)
Key features and details
- Mouse monoclonal [2782C5] to Rad6
- Suitable for: IHC-M, WB
- Isotype: IgG2a
Overview
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Product name
Anti-Rad6 antibody [2782C5]
See all Rad6 primary antibodies -
Description
Mouse monoclonal [2782C5] to Rad6 -
Host species
Mouse -
Tested applications
Suitable for: IHC-M, WBmore details -
Immunogen
Recombinant fragment corresponding to Human Rad6 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2782C5 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-M |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
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Notes |
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IHC-M
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Alternative names
- BHR6A antibody
- hHR6A antibody
- HR6A antibody
see all
Images
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Anti-Rad6 antibody [2782C5] (ab50889) + immunizing peptide
Predicted band size: 17 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab50889 has not yet been referenced specifically in any publications.