Key features and details
- Mouse monoclonal [2782C5] to Rad6
- Suitable for: IHC-M, WB, Dot blot
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Rad6 antibody [2782C5]
See all Rad6 primary antibodies
DescriptionMouse monoclonal [2782C5] to Rad6
Tested applicationsSuitable for: IHC-M, WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Rad6 (internal sequence).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- E2 Ubiquitin Conjugating Enzymes
Our Abpromise guarantee covers the use of ab50889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 17 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- BHR6A antibody
- hHR6A antibody
- HR6A antibody
Anti-Rad6 antibody [2782C5] (ab50889) + immunizing peptide
Predicted band size: 17 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab50889 has not yet been referenced specifically in any publications.