Overview

  • Product name

  • Description

    Rabbit polyclonal to Rad6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Mmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-141 of Human Rad6 (Uniprot ID: P49459).

  • Positive control

    • Mouse brain whole cell lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab155001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
ICC/IF 1/100 - 1/1000.
IHC-M Use at an assay dependent concentration.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links

  • Alternative names

    • BHR6A antibody
    • hHR6A antibody
    • HR6A antibody
    • mHR6A antibody
    • MRXS30 antibody
    • MRXSN antibody
    • RAD6 homolog A antibody
    • RAD6A antibody
    • UBC2 antibody
    • UBCD6 antibody
    • UBE2A antibody
    • Ube2a ubiquitin-conjugating enzyme E2A antibody
    • UBE2A_HUMAN antibody
    • Ubiquitin carrier protein A antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 17 kDa antibody
    • Ubiquitin conjugating enzyme E2 21.5 kDa antibody
    • Ubiquitin conjugating enzyme E2A (RAD6 homolog) antibody
    • Ubiquitin conjugating enzyme E2A antibody
    • Ubiquitin protein ligase A antibody
    • Ubiquitin-conjugating enzyme E2 A antibody
    • Ubiquitin-protein ligase A antibody
    see all

Images

  • Anti-Rad6 antibody (ab155001) at 1/500 dilution + Mouse brain (whole cell lysate) at 50 µg

    Predicted band size: 17 kDa



    15% SDS PAGE
  • Immunofluorescent analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, labeling Rad6 (Green) with ab155001 at 1/500. Second image shows costaining with Hoechst 33342.

References

ab155001 has not yet been referenced specifically in any publications.

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