Key features and details
- Rabbit polyclonal to Rad6
- Suitable for: IHC-P, IHC-M
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Rad6 antibody
See all Rad6 primary antibodies
DescriptionRabbit polyclonal to Rad6
Tested applicationsSuitable for: IHC-P, IHC-Mmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human Rad6 (N terminal). Synthetic peptide from the N-terminal of Human Rad6, Swiss-Prot P49459.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- E2 Ubiquitin Conjugating Enzymes
Our Abpromise guarantee covers the use of ab84395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. Likely to work in frozen sections.|
|IHC-M||Use at an assay dependent concentration.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- BHR6A antibody
- hHR6A antibody
- HR6A antibody
Detection of Human Rad6 by Immunohistochemistry.
Sample: FFPE section of Human stomach adenocarcinoma.Antibody: ab84395 used at a dilution of 1/250.
Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.
Detection of Mouse Rad6 by Immunohistochemistry.
Sample: FFPE section of Mouse squamous cell carcinoma.Antibody: ab84395 used at a dilution of 1/250.
Detection: DAB staining using anti Rabbit IHC antibody at a dilution of 1/100.
ab84395 has been referenced in 1 publication.
- Read ML et al. The PTTG1-binding factor (PBF/PTTG1IP) regulates p53 activity in thyroid cells. Endocrinology 155:1222-34 (2014). PubMed: 24506068