Recombinant Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1862Y] to Radixin/RDX
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Radixin/RDX antibody [EP1862Y]
See all Radixin/RDX primary antibodies -
Description
Rabbit monoclonal [EP1862Y] to Radixin/RDX -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Radixin/RDX aa 350-450 (N terminal). The exact sequence is proprietary.
-
Positive control
- WB: HeLa cell lysate. IHC-P: Paraffin embedded human ovarian carcinoma. Flow Cyt (intra): HeLa cells. ICC/IF: Mcf7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1862Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (2) |
Use a concentration of 5 µg/ml.
|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane. -
Involvement in disease
Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Contains 1 FERM domain. -
Domain
The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively. - Information by UniProt
-
Database links
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 315655 Rat
- Omim: 179410 Human
- SwissProt: P35241 Human
- SwissProt: P26043 Mouse
- Unigene: 263671 Human
- Unigene: 592679 Human
see all -
Alternative names
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
Images
-
Anti-Radixin/RDX antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (22)
ab52495 has been referenced in 22 publications.
- Seker U et al. Effects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liver. Hepatol Forum 3:21-26 (2022). PubMed: 35782372
- Zhang Y et al. Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice. Nat Commun 12:2523 (2021). PubMed: 33953177
- Zhang L et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
- Zhang L et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313
- Zhang N et al. LncRNA linc01116 prometes glioma cell migration and invasion by modulation of radixin targeted by miR-31. Int J Clin Exp Pathol 12:1078-1086 (2019). PubMed: 31933922