Key features and details
- Rabbit polyclonal to Radixin/RDX
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Radixin/RDX antibody
See all Radixin/RDX primary antibodies
DescriptionRabbit polyclonal to Radixin/RDX
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Pig
Recombinant fragment within Human Radixin/RDX (internal sequence). The exact sequence is proprietary.
Database link: P35241
- IHC-P: Rat liver tissue. WB: HepG2 whole cell lysate.
This product was previously labelled as Radixin
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 69 kDa.|
FunctionProbably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Involvement in diseaseDefects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesContains 1 FERM domain.
DomainThe N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
modificationsPhosphorylated by tyrosine-protein kinases.
Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
- Information by UniProt
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
All lanes : Anti-Radixin/RDX antibody (ab227266) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Radixin/RDX Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 69 kDa
Anti-Radixin/RDX antibody (ab227266) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 69 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded rat liver tissue stained for Radixin/RDX using ab227266 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227266 has not yet been referenced specifically in any publications.