Recombinant
RabMAb

Recombinant Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

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Overview

  • Product name

    Anti-Radixin/RDX antibody [EP1862Y]
    See all Radixin/RDX primary antibodies

  • Description

    Rabbit monoclonal [EP1862Y] to Radixin/RDX

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Radixin/RDX aa 350-450 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate IHC-P: Paraffin embedded human ovarian carcinoma

  • General notes

     This product was previously labelled as Radixin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1862Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IP 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

  • Involvement in disease

    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

  • Sequence similarities

    Contains 1 FERM domain.

  • Domain

    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.

  • Post-translational
    modifications

    Phosphorylated by tyrosine-protein kinases.

  • Cellular localization

    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

Images

  • Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Anti-Radixin/RDX antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
    Flow Cytometry - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)

    Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:

  • Ou R  et al. HPV16 E7-induced upregulation of KDM2A promotes cervical cancer progression by regulating miR-132-radixin pathway. J Cell Physiol 234:2659-2671 (2019). Read more (PubMed: 30132864) »
  • Magnin E  et al. Input-Specific Synaptic Location and Function of the a5 GABAA Receptor Subunit in the Mouse CA1 Hippocampal Neurons. J Neurosci 39:788-801 (2019). Read more (PubMed: 30523065) »
See all 15 Publications for this product

Publishing research using ab52495? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-Radixin antibody [EP1862Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
30 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

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Submitted Mar 27 2013

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