Recombinant Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
![Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)](https://www.abcam.com/ps/products/52/ab52495/Images/ab52495-64770-anti-radixin-antibody-ep1862y-western-blot.jpg "Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1862Y] to Radixin/RDX
- Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Radixin/RDX antibody [EP1862Y]
See all Radixin/RDX primary antibodies -
Description
Rabbit monoclonal [EP1862Y] to Radixin/RDX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Radixin/RDX aa 350-450 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa cell lysate. IHC-P: Paraffin embedded human ovarian carcinoma. Flow Cyt: HeLa cells. ICC/IF: Mcf7 cells.
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General notes
Radixin/RDX antibody (ab52495) was previously labelled as Radixin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1862Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
|
| Flow Cyt |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). |
|
Flow Cyt
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane. -
Involvement in disease
Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Contains 1 FERM domain. -
Domain
The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively. - Information by UniProt
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Database links
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 315655 Rat
- Omim: 179410 Human
- SwissProt: P35241 Human
- SwissProt: P26043 Mouse
- Unigene: 263671 Human
- Unigene: 592679 Human
see all -
Alternative names
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
Images
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Western blot - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)Anti-Radixin/RDX antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)](https://www.abcam.com/ps/products/52/ab52495/Images/ab52495-64769-anti-radixin-antibody-ep1862y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)](https://www.abcam.com/ps/products/52/ab52495/Images/ab52495-64768-anti-radixin-antibody-ep1862y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)](https://www.abcam.com/ps/products/52/ab52495/Images/ab52495-64767-anti-radixin-antibody-ep1862y-flow-cytometry.jpg)
Flow Cytometry - Anti-Radixin/RDX antibody [EP1862Y] (ab52495)Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Anti-Radixin/RDX antibody [EP1862Y] (ab52495)](https://www.abcam.com/ps/products/52/ab52495/Images/ab52495-5-benefits-of-recombinant-antibodies.png)
Anti-Radixin/RDX antibody [EP1862Y] (ab52495)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab52495 has been referenced in 16 publications.
- Ou R et al. HPV16 E7-induced upregulation of KDM2A promotes cervical cancer progression by regulating miR-132-radixin pathway. J Cell Physiol 234:2659-2671 (2019). PubMed: 30132864
- Magnin E et al. Input-Specific Synaptic Location and Function of the a5 GABAA Receptor Subunit in the Mouse CA1 Hippocampal Neurons. J Neurosci 39:788-801 (2019). PubMed: 30523065
- Mu L et al. A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing. Nat Commun 9:4259 (2018). PubMed: 30323235
- Chen H & Dai J miR-409-3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX. Oncol Lett 16:543-551 (2018). PubMed: 29928443
- Yoshida S et al. Comprehensive proteome analysis of brush border membrane fraction of ileum of ezrin knockdown mice. Biomed Res 37:127-39 (2016). PubMed: 27108882