Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6923] to RAE1
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-RAE1 antibody [EPR6923]
See all RAE1 primary antibodies
DescriptionRabbit monoclonal [EPR6923] to RAE1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human RAE1 aa 300-400. The exact sequence is proprietary.
- MCF7, HeLa, HepG2, 293T and SH-SY5Y cell lysates; Human kidney tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min|
FunctionBinds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
Sequence similaritiesBelongs to the WD repeat rae1 family.
Contains 4 WD repeats.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- dJ481F12.3 antibody
- dJ800J21.1 antibody
- FLJ30608 antibody
All lanes : Anti-RAE1 antibody [EPR6923] (ab124783) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
ab124783 at 1/100 dilution staining RAE1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124783 has not yet been referenced specifically in any publications.