Overview

  • Product name

  • Description

    Rabbit polyclonal to Raf1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse Raf1 aa 86-113 (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    LKVRGLQPECCAVFRLLQEHKGKKARLD


    Database link: Q99N57

  • Positive control

    • WB: Neuro-2a and Jurkat whole cell lysate (ab7899). IHC-P: Human skin tissue. Flow: Neuro-2a cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 73 kDa.
Flow Cyt 1/10 - 1/50.
IHC-P 1/100.

Target

  • Function

    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • Tissue specificity

    In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • Involvement in disease

    Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • c Raf antibody
    • C-raf antibody
    • C-Raf proto-oncogene, serine/threonine kinase antibody
    • CMD1NN antibody
    • Craf 1 transforming gene antibody
    • cRaf antibody
    • Craf1 transforming gene antibody
    • EC 2.7.11.1 antibody
    • kinase Raf1 antibody
    • Murine sarcoma 3611 oncogene 1 antibody
    • NS5 antibody
    • Oncogene MIL antibody
    • Oncogene RAF1 antibody
    • OTTHUMP00000160218 antibody
    • OTTHUMP00000207813 antibody
    • OTTHUMP00000209389 antibody
    • Protein kinase raf 1 antibody
    • Proto-oncogene c-RAF antibody
    • Raf 1 antibody
    • Raf 1 proto oncogene serine/threonine kinase antibody
    • RAF antibody
    • Raf proto oncogene serine/threonine protein kinase antibody
    • RAF proto-oncogene serine/threonine-protein kinase antibody
    • RAF-1 antibody
    • RAF1 antibody
    • RAF1_HUMAN antibody
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    • v raf 1 murine leukemia viral oncogene homolog 1 antibody
    • v-raf murine sarcoma viral oncogene homolog 1 antibody
    • v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
    • vraf1 murine leukemia viral oncogene homolog 1 antibody
    see all

Images

  • Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Raf1 using ab230850 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Flow cytometric analysis of Neuro-2a (mouse neuroblastoma cell line) cell line (right histogram) labeling Raf1 with ab230850 at 1/10 dilution compared with a negative control cell line (left histogram). FITC-conjugated donkey anti-rabbit secondary antibodies were used for the analysis.

References

ab230850 has not yet been referenced specifically in any publications.

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