Anti-Raf1 antibody (ab230850)
Key features and details
- Rabbit polyclonal to Raf1
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse Raf1 aa 86-113 (N terminal) conjugated to keyhole limpet haemocyanin.
Sequence:LKVRGLQPECCAVFRLLQEHKGKKARLD
Database link: Q99N57 -
Positive control
- WB: Neuro-2a and Jurkat whole cell lysate (ab7899). IHC-P: Human skin tissue. Flow: Neuro-2a cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab230850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 73 kDa. | |
| Flow Cyt | 1/10 - 1/50. | |
| IHC-P | 1/100. |
Target
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Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
Involvement in disease
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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Database links
- Entrez Gene: 521196 Cow
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 100173732 Orangutan
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: A7E3S4 Cow
- SwissProt: P04049 Human
see all -
Alternative names
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
Images
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Western blot - Anti-Raf1 antibody (ab230850)Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab230850)Formalin-fixed, paraffin-embedded human skin tissue stained for Raf1 using ab230850 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-Raf1 antibody (ab230850)Anti-Raf1 antibody (ab230850) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 73 kDa -
Flow Cytometry - Anti-Raf1 antibody (ab230850)Flow cytometric analysis of Neuro-2a (mouse neuroblastoma cell line) cell line (right histogram) labeling Raf1 with ab230850 at 1/10 dilution compared with a negative control cell line (left histogram). FITC-conjugated donkey anti-rabbit secondary antibodies were used for the analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab230850 has been referenced in 1 publication.
- Wang S et al. Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. BMC Cancer 19:1160 (2019). PubMed: 31783811
