• Product name

    Anti-Raf1 (phospho S621) antibody [EPR1521(2)]
    See all Raf1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1521(2)] to Raf1 (phospho S621)
  • Host species

  • Specificity

    This antibody only detects Raf1 when phosphorylated at serine 621.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues surrounding serine 621 of Human Raf1 (Uniprot: P04049)

  • Positive control

    • HeLa cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab157201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
    • Tissue specificity

      In skeletal muscle, isoform 1 is more abundant than isoform 2.
    • Involvement in disease

      Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
      Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 RBD (Ras-binding) domain.
    • Post-translational

      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
    • Cellular localization

      Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • c Raf antibody
      • C-raf antibody
      • C-Raf proto-oncogene, serine/threonine kinase antibody
      • CMD1NN antibody
      • Craf 1 transforming gene antibody
      • cRaf antibody
      • Craf1 transforming gene antibody
      • EC antibody
      • kinase Raf1 antibody
      • Murine sarcoma 3611 oncogene 1 antibody
      • NS5 antibody
      • Oncogene MIL antibody
      • Oncogene RAF1 antibody
      • OTTHUMP00000160218 antibody
      • OTTHUMP00000207813 antibody
      • OTTHUMP00000209389 antibody
      • Protein kinase raf 1 antibody
      • Proto-oncogene c-RAF antibody
      • Raf 1 antibody
      • Raf 1 proto oncogene serine/threonine kinase antibody
      • RAF antibody
      • Raf proto oncogene serine/threonine protein kinase antibody
      • RAF proto-oncogene serine/threonine-protein kinase antibody
      • RAF-1 antibody
      • RAF1 antibody
      • RAF1_HUMAN antibody
      • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
      • v raf 1 murine leukemia viral oncogene homolog 1 antibody
      • v-raf murine sarcoma viral oncogene homolog 1 antibody
      • v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
      • vraf1 murine leukemia viral oncogene homolog 1 antibody
      see all


    • All lanes : Anti-Raf1 (phospho S621) antibody [EPR1521(2)] (ab157201) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa


    ab157201 has not yet been referenced specifically in any publications.

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