Overview

  • Product name

    Anti-Rag A antibody
  • Description

    Rabbit polyclonal to Rag A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Mammals
  • Immunogen

    Recombinant full length protein corresponding to Human Rag A.
    Database link: Q7L523

  • Positive control

    • HeLa cell lysate.
  • General notes

    Previously labelled as RRAGA.

Properties

Applications

Our Abpromise guarantee covers the use of ab128196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 37 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
  • Tissue specificity

    Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
  • Sequence similarities

    Belongs to the GTR/RAG GTP-binding protein family.
  • Cellular localization

    Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state. Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenovirus E3 14.7 kDa-interacting protein 1 antibody
    • FIP-1 antibody
    • Rag A antibody
    • RagA antibody
    • Ras-related GTP-binding protein A antibody
    • RRAGA antibody
    • RRAGA_HUMAN antibody
    see all

Images

  • All lanes : Anti-Rag A antibody (ab128196) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : MOLT4 cell lysate
    Lane 4 : NIH-3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 37 kDa

  • Anti-Rag A antibody (ab128196) at 1/1000 dilution + HeLa whole cell lysate at 10 µg

    Predicted band size: 37 kDa

  • HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-Rag A antibody at 1/100 dilution. As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right.

References

ab128196 has not yet been referenced specifically in any publications.

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