Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
Use PBS/EDTA to detach cells to preserve the glycoproteins on the cell surface; do not fix, do not permeabilise.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ELISA||1/2000 - 1/10000.|
FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Tissue specificityEndothelial cells.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- MGC2235 antibody
All lanes : Anti-RAGE antibody (ab37647) at 1/500 dilution
Lane 1 : HUVEC whole cell lysate
Lane 2 : EVC304 whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 17,40 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The band at 52kDa is a tubulin loading control.
RAW 264.7 cells were stained with anti-RAGE (1ug/million cells) for 1hr at 4 deg C followed by staining with anti-rabbit IgG-PE conjugate. Cells were analyzed by flow cytometry. Unstained cells or cells stained with secondary antibody alone are represented in the background
ab37647 at a 1/100 dilution staining RAGE in bovine endothelial cells by Immunocytochemistry/ Immunofluorescence. Fixed in PFA, permeabilized with Triton X-100. Blocked using 10% serum for 20 minutes at room temperature. Secondary used at 1/200 polyclonal Goat snti-rabbit conjugated to Alexa Fluor 568 (red). Nuclear staining (blue).
All lanes : Anti-RAGE antibody (ab37647) at 1/2000 dilution
Lane 1 : Bovine lung extract
Lane 2 : Mouse lung extract
Lane 3 : HeLa cells expressing human soluble RAGE
All lanes : anti-rabbit IgG alkaline phosphatase conjugate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Bovine RAGE is slightly larger than human and mouse RAGE - 44kDa compared with 42kDa
ab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.
Specimen tube 006 is negative control
ICC/IF image of ab37647 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Wu DM et al. S100A9 gene silencing inhibits the release of pro-inflammatory cytokines by blocking the IL-17 signalling pathway in mice with acute pancreatitis. J Cell Mol Med 22:2378-2389 (2018). WB ; Mouse . Read more (PubMed: 29441717) »
- Jandial R et al. Inhibition of GLO1 in Glioblastoma Multiforme Increases DNA-AGEs, Stimulates RAGE Expression, and Inhibits Brain Tumor Growth in Orthotopic Mouse Models. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29385725) »