Anti-RAGE antibody (ab65965)
Key features and details
- Rabbit polyclonal to RAGE
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-RAGE antibody
See all RAGE primary antibodies -
Description
Rabbit polyclonal to RAGE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.
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Positive control
- Rat lung tissue and lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Rat
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| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IHC-P |
| Notes |
|---|
IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 81722 Rat
- SwissProt: Q63495 Rat
- Unigene: 9829 Rat
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Alternative names
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab65965)Immunohistochemical analysis of paraffin-embedded Rat lung tissue sections using ab65965 at 1µg/ml
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Western blot - Anti-RAGE antibody (ab65965)Anti-RAGE antibody (ab65965) at 2 µg/ml + Rat lung tissue lysate
Predicted band size: 48 kDa
Observed band size: 48 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab65965 has been referenced in 3 publications.
- Chen YY et al. Dapagliflozin Prevents NOX- and SGLT2-Dependent Oxidative Stress in Lens Cells Exposed to Fructose-Induced Diabetes Mellitus. Int J Mol Sci 20:N/A (2019). PubMed: 31491943
- Lai S et al. Glycyrrhizin Treatment Facilitates Extinction of Conditioned Fear Responses After a Single Prolonged Stress Exposure in Rats. Cell Physiol Biochem 45:2529-2539 (2018). PubMed: 29558743
- Wang H et al. Expression of the receptor for advanced glycation end-products and frequency of polymorphism in lung cancer. Oncol Lett 10:51-60 (2015). IHC-P ; Human . PubMed: 26170976