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    rage-antibody-ab65965.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid
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Anti-RAGE antibody (ab65965)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab65965)
  • Western blot - Anti-RAGE antibody (ab65965)

Key features and details

  • Rabbit polyclonal to RAGE
  • Suitable for: WB, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human RAGE protein (Fc Chimera Active) (ab219875)

View more associated products

Overview

  • Product name

    Anti-RAGE antibody
    See all RAGE primary antibodies
  • Description

    Rabbit polyclonal to RAGE
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.

  • Positive control

    • Rat lung tissue and lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Sensory System
    • Visual system
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human RAGE protein (Fc Chimera Active) (ab219875)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
WB
Rat
Application Abreviews Notes
WB
IHC-P
Notes
  • Application notes
    ICC: Use at a concentration of 0.5 - 1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
    • Tissue specificity

      Endothelial cells.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q15109 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 81722 Rat
      • SwissProt: Q63495 Rat
      • Unigene: 9829 Rat
      • Alternative names

        • Advanced glycosylation end product-specific receptor antibody
        • Ager antibody
        • DAMA 358M23.4 antibody
        • MGC2235 antibody
        • MGC22357 antibody
        • RAGE_HUMAN antibody
        • Receptor for advanced glycation end products antibody
        • Receptor for advanced glycosylation end products antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab65965)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab65965)
        Immunohistochemical analysis of paraffin-embedded Rat lung tissue sections using ab65965 at 1µg/ml
      • Western blot - Anti-RAGE antibody (ab65965)
        Western blot - Anti-RAGE antibody (ab65965)
        Anti-RAGE antibody (ab65965) at 2 µg/ml + Rat lung tissue lysate

        Predicted band size: 48 kDa
        Observed band size: 48 kDa

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab65965? Please let us know so that we can cite the reference in this datasheet.

      ab65965 has been referenced in 3 publications.

      • Chen YY  et al. Dapagliflozin Prevents NOX- and SGLT2-Dependent Oxidative Stress in Lens Cells Exposed to Fructose-Induced Diabetes Mellitus. Int J Mol Sci 20:N/A (2019). PubMed: 31491943
      • Lai S  et al. Glycyrrhizin Treatment Facilitates Extinction of Conditioned Fear Responses After a Single Prolonged Stress Exposure in Rats. Cell Physiol Biochem 45:2529-2539 (2018). PubMed: 29558743
      • Wang H  et al. Expression of the receptor for advanced glycation end-products and frequency of polymorphism in lung cancer. Oncol Lett 10:51-60 (2015). IHC-P ; Human . PubMed: 26170976

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Which part of the rat lung was stained in the IHC-P image?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 31 2012

      Answer

      Thank you for your enquiry.

      I am sorry that the image is not very clear. Please rest assured that the positive staining was in the bronchiole respiratory epithelium. I have attached a simlar image found in the following histology book for your reference.

      Anthony Mescher. 2010. Junqueira's Basic Histology (Text & Atlas) 12th Edition. McGraw Hill. Pg 307.

      I hope this information will be helpful.

      Read More

      Abcam Scientific Support

      Answered on Aug 31 2012

      Question

      anti RAGE AK fuer Kaninchenproben in WB und IHC-P?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 23 2012

      Answer

      Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

      Ich habe zwei Antikörper gefunden, deren Immunogene eine hohe Homologie mit Kaninchen aufweisen und die schon für WB und IHC-P getestet wurden:

      ab65965: 95%

      https://www.abcam.com/RAGE-antibody-ab65965.html (or use the following: https://www.abcam.com/RAGE-antibody-ab65965.html).

      ab86767: 96%

      https://www.abcam.com/RAGE-antibody-ab86767.html (or use the following: https://www.abcam.com/RAGE-antibody-ab86767.html).



      Unserem Wissen nach wurden wederab65965 noch ab86767 bisher im Kaninchen getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von dem getesteten Antikörper abdecken, und gegen eine erneute Bestellung eines weiterenprimären Antikörpersvon uns verrechnet werden.

      Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

      1.) Bestätigen Sie mir bitte, dasswelchen Antikörper SieimKaninchen testen möchten.

      2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

      3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

      4.) Testen Sie den Antikörper im Kaninchen.

      5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
      Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
      https://www.abcam.com/abreviews

      6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderenprimären Antikörperzu dem gleichen Preis wieden getestetenbei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

      Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

      Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
      https://www.abcam.com/collaborationdiscount

      Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

      Read More

      Abcam Scientific Support

      Answered on Feb 23 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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