Key features and details
- Rabbit polyclonal to RAGE
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-RAGE antibody
See all RAGE primary antibodies
DescriptionRabbit polyclonal to RAGE
Tested Applications & Species
Application Species IHC-PRat WBRat
A synthetic peptide corresponding to a sequence mapping in the middle region of human advanced glycosylation end-product-specific receptor(RAGE), different from the mouse and rat sequence by two amino acids.
- Rat lung tissue and lysate
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FormLyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Tissue specificityEndothelial cells.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
ab65965 has been referenced in 3 publications.
- Chen YY et al. Dapagliflozin Prevents NOX- and SGLT2-Dependent Oxidative Stress in Lens Cells Exposed to Fructose-Induced Diabetes Mellitus. Int J Mol Sci 20:N/A (2019). PubMed: 31491943
- Lai S et al. Glycyrrhizin Treatment Facilitates Extinction of Conditioned Fear Responses After a Single Prolonged Stress Exposure in Rats. Cell Physiol Biochem 45:2529-2539 (2018). PubMed: 29558743
- Wang H et al. Expression of the receptor for advanced glycation end-products and frequency of polymorphism in lung cancer. Oncol Lett 10:51-60 (2015). IHC-P ; Human . PubMed: 26170976