Overview

  • Product name

    Anti-RAGE antibody [EPR12205]
    See all RAGE primary antibodies
  • Description

    Rabbit monoclonal [EPR12205] to RAGE
  • Host species

    Rabbit
  • Specificity

    Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RAGE aa 1-100. The exact sequence is proprietary.
    Database link: Q15109

  • Positive control

    • Fetal lung and mouse lung lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
    • Tissue specificity

      Endothelial cells.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • DAMA 358M23.4 antibody
      • MGC2235 antibody
      • MGC22357 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycation end products antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    Images

    • All lanes : Anti-RAGE antibody [EPR12205] (ab181369) at 1/1000 dilution

      Lane 1 : Mouse lung lysates with 5% NFDM/TBST
      Lane 2 : Mouse brain lysates with 5% NFDM/TBST
      Lane 3 : Mouse kidney lysates with 5% NFDM/TBST
      Lane 4 : Mouse heart lysates with 5% NFDM/TBST
      Lane 5 : Mouse liver lysates with 5% NFDM/TBST
      Lane 6 : Mouse spleen lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size: 42 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      The expression profile and molecular mass observed is consistent with what has been described in the literature (PMID:16315007; PMID:18355449; PMID:18245812)

    • All lanes : Anti-RAGE antibody [EPR12205] (ab181369) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Mouse lung lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa

    References

    ab181369 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181369.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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